The Functional Proteomics Facility (FPF) is equipped with instrumentation for the fractionation of complex biological mixtures as well as instrumentation for the subsequent analysis and processing of these samples in preparation for analysis by mass spectrometry.
Instrumentation housed within this facility includes:
Researchers may conduct their own experiments after completing the appropriate training, or, submit samples and the Facility Manager will conduct experiments on a fee-for-service basis.
The Functional Proteomics Facility at Western University was established in 2005, in order to provide the scientific community with a number of different instrumentation to aid in complex biological sample analysis. The Facility offers various tools to perform protein purification, chromatographic and/or electrophoretic separations, gel imaging and image analysis, robotic spot picking, and automated in-gel digestion.
The sample preparation carried out at The Functional Proteomics Facility is particularly suited for high-throughput protein identification by mass spectrometry. To offer a direct service of protein identification by peptide mass fingerprint, the Facility works in close conjunction with the MALDI Mass Spectrometry Facility.