Cancer Research Trainee Spotlight
Western University is home to many talented research trainees who conduct research across various city-wide university and hospital facilities. They play an integral part in oncology research.
The featured trainees are all in various stages of training and were randomly selected amongst the members of the Centre for Translational Cancer Research, who act as mentors and supervisors throughout their training. The trainees were asked to complete the following questions.
What is your undergraduate/master's degree and from which institution:
Rober Abdo *(Jan/25, rabdo3@uwo.ca)
Doctor of Medicine (M.D.), The University of Aleppo, Syria; Master of Medical Science, The University of Western Ontario.
4th year PhD Candidate, Pathology & Laboratory Department. Supervisor: Qi Zhang MD PhD, London Health Sciences Centre and Schulich School of Medicine & Dentistry, and Shun-Cheng LI PhD, London, Western University
Project: My research focuses on understanding the molecular and cellular mechanisms driving brain metastases, with a current emphasis on those originating from breast cancer. Using state-of-the-art techniques, I aim to uncover how cancer cells adapt to the brain's unique environment and identify potential therapeutic targets to prevent or treat these metastases. This work has the potential to significantly improve the outcomes for patients suffering from this challenging condition.
Key Techniques Used: I utilize advanced single-cell RNA sequencing, spatial transcriptomics, proteomics and phosphoproteomics to map gene expression at a cellular level. By combining bioinformatics with in-vivo validation, I decode complex molecular interactions, enabling precise identification of potential therapeutic targets and understanding tumor-stromal cell interactions.
Why this project?: Brain metastases remain one of the most devastating complications of cancer, yet they are poorly understood. I chose this project to contribute to advancing our knowledge and developing targeted therapies that could bring hope to patients and families facing this condition.
Why Western?: Western University provides a supportive research environment with cutting-edge facilities and renowned expertise in cancer research, making it an ideal place to pursue my PhD.
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Ryan Au *(Jan/25, rau23@uwo.ca)
BSc. in Medical Physics, McMaster's University and MSc. in Physics, Toronto Metropolitan University
4th year PhD Candidate in Medical Biophysics, Supervisor: Aaron Ward PhD and Glenn Bauman MD (Baines Imaging Research Laboratories, VFCC)
Project: My research focuses on developing different tools that could help radiologists more accurately identify prostate cancer on magnetic resonance imaging (MRI). MRI is the key imaging modality used to diagnose prostate cancer, but small cancers may be missed by radiologists. Therefore, we are using eye tracking technology to understand how radiologists currently use MRIs to identify prostate cancer, and identify specific search patterns that lead to more accurate cancer identifications. We are also using artificial intelligence to map cancers seen on high resolution images of prostate tissue onto MRIs so that radiologists can better understand how MRI findings relate to the underlying tissue.
Key Techniques Used: magnetic resonance imaging, digital whole-mount histopathology, generative artificial intelligence, and remote eye tracking.
Why this project?: I am very interested in developing and implementing various tools that would be beneficial in clinical settings. This project allows me to pursue these interests by developing novel artificial intelligence systems and bringing eye tracking technology (commonly used in computer gaming) into the medical setting to ultimately improve clinical workflows and enable more accurate cancer diagnoses.
Why Western?: Dr. Ward's research program provided a unique opportunity to work within a cancer centre where I could get immediate input and feedback from radiation oncologists. Having the privilege of being so close to the frontlines ensures everything I am developing will be useful within the clinic.
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Natasha Sophia Bruce *(Jan/24, nbruce9@uwo.ca)
MSc. Research Biology in Anatomy and Cell Biology
1st year Masters Candidate in Anatomy and Cell Biology, Supervisor: Trevor Shepherd PhD.
My Project: My research focuses on understanding ovarian cancer spread and overcoming chemotherapy resistance. We identified LKB1 as a critical molecule that supports ovarian cancer cell survival and spread. In partnership with the Ontario Institute for Cancer Research, we are developing drugs to inhibit LKB1. My project tests these drugs on ovarian cancer cells and patient samples to evaluate their ability to reduce cell viability and metastasis. Ultimately, we aim to combine these LKB1 inhibitors with chemotherapy to improve outcomes for women with ovarian cancer.
Key Techniques Used: Drug sensitivity assay, Cell viability, Western blotting, Reattachment assays and in general a lot of cell culture work.
Why this project?:I have always been personally invested in ovarian cancer research, so when the opportunity to work on this project arose, I knew I couldn’t pass it up.
Why Western?: I chose to go to Western University because of its excellent research opportunities.
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Sylvia Lin Cheng *(March/25, sche243@uwo.ca)
Bachelors of Medical Sciences, Specialization in Biochemistry and Cancer Biology, Schulich School of Medicine & Dentistry, Western University
Tiffany Cheung, *(March /25, tcheun89@uwo.ca)
BMSc in Medical Sciences, Schulich School of Medicine & Dentistry, Western University
1st year, Master's Candidate, Department of Pathology & Laboratory Medicine, Schulich School of Medicine and Dentistry. Supervisor: Saman Maleki PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Cancer immunotherapy targets the immune system to attack cancer cells, but multiple factors affect a patient's response to this treatment. The gut microbiome is the collection of microorganisms that reside in our gut, and its composition can influence immunotherapy response by modulating immune responses. Antibiotic use, which disrupts the gut microbiome, has been linked to worsened treatment outcomes in immunotherapy patients. My research aims to investigate the effect of antibiotics on systemic and local immune responses within the tumour environment and to identify bacterial species driving tumour growth.
Key Techniques Used: I conduct metagenomics analysis on stool samples collected from tumour-bearing mice previously treated with antibiotics to study how antibiotics modulate their gut microbiomes. I also use flow cytometry analysis to identify antibiotic-driven changes in the immune cell population within the tumour and systemically.
Why this project?: I have always wanted to contribute to cancer research to drive meaningful change, and studying how the gut microbiome influences anti-cancer and immunotherapy responses is a novel and exciting approach to improving existing cancer treatments. This interdisciplinary project allows me to learn and advance a variety of skills.
Why Western?: I chose Western University for its strong cancer research program as it offers unique interdisciplinary opportunities to engage in translational research.
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Chloe Davidson *(April/25) cdavid83@uwo.ca
BSc in Health Sciences, Wilfrid Laurier University.
1st year MSc Candidate, Department of Pathology & Laboratory Medicine, Schulich School of Medicine and Dentistry. Supervisor: Saman Maleki PhD, Department of Oncology, Medical Biophysics, Pathology & Laboratory Medicine, Schulich School of Medicine & Dentistry, Western University. Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre Research Institute.
Project: The DNA mismatch repair (MMR) pathway corrects errors that occur during DNA replication. A deficiency in this pathway increases mutational burden within the tumour microenvironment, making them more susceptible to anti-tumour immune responses. My research looks at the differences between MMR-deficient colorectal cancer tumours, specifically interested in dendritic cells and T-cells which are key immune cells that facilitate an immune response within the body. Looking at differential maturation and activation status of these cells within the tumour microenvironment can give us insight as to why some MMR-deficient tumours respond to immunotherapy, while others do not.
Key Techniques Used: Flow cytometry, cell co-cultures, bone marrow extraction, mouse handling
Why this Project?: I have always had an interest in cancer research. This project allows me to explore the relationship between immune responses and cancer, where I will hopefully improve treatment outcomes in patients through the use of immunotherapy.
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Mackenzie Dodge, *(March/25, mdodge@uwo.ca)
BMSc in Microbiology and Immunology, Department of Microbiology & Immunology, Schulich School of Medicine & Dentistry, Western University.
7th Year, PhD Candidate, Department of Microbiology & Immunology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Joe Mymryk PhD, Cancer Reseach Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research focuses on human adenovirus - a ubiquitous human pathogen that can cause severe illness in immunocompromised people. Specifically, I study how adenovirus interacts with our cells during infection, looking at tricks the virus uses that may be shared by other viruses. I am also looking into new treatment options for severe adenovirus infections.
Key Techniques Used?: Cell culture, luciferase assays, western blotting, co-immunoprecipitation, qPCR.
Why this Project?: I chose this project because it gave me the opportunity to explore and hone a variety of different techniques, specifically helping me improve my molecular biology and virology skills.
Why Western?: I chose Western because I was familiar with the Microbiology and Immunology program after completing my undergraduate studies here, and I felt that I would learn a lot from my supervisor and his research program.
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Nitara Fernando *(Dec/24, nferna47@uwo.ca)
BSc. in Medical and Biological Physics, McMaster University
3rd year PhD Candidate in Department of Medical Biophysics, Supervisor: Paula Foster PhD, Imaging Pathogens for Knowledge Translation (ImPaKT) Facility, Western University
Project: My research is focused on the development of Magnetic Particle Imaging (MPI) for imaging of cancer metastasis. Metastasis causes over 90% of tumour-related deaths, often spreading first to nearby lymph nodes. The sentinel lymph node (SLN) is the first node or group of nodes cancer will spread to; thus, its detection is crucial for determining cancer spread and guiding treatment. Current SLN mapping uses a Technetium (99m-Tc) tracer and blue dye, but these have drawbacks like short tracer half-life and radiation exposure. Magnetic SLN localization with superparamagnetic iron oxide (SPIO) particles avoids radiation and has a longer lifespan, however, it currently relies on a hand-held magnetometer during biopsy and does not allow for preoperative imaging. We propose developing MPI Lymphography, a new imaging technique using MPI to directly detect SPIOs, providing clear and quantifiable SLN imaging before surgery. This project aims to optimize MPI SLN imaging in mice prior to its clinical application.
Key techniques used: cell culture, mouse handling/injections, MPI imaging, Horos image analysis, histology
Why this project?: This project was very exciting to me because of its novelty and potential clinical impact. Investigating MPI lymphography in preclinical models is a critical step toward translating this technology to clinical practice. Our collaborators at Magnetic Insight are translating MPI to the clinic by engineering the first human-sized head and neck MPI imager and we can anticipate that MPI will be used in patients in the near future!
Why Western?: I chose to pursue my PhD at Western University's Medical Biophysics Department because of its interdisciplinary research opportunities at the intersection of physics, biology, and engineering. In the Foster Lab, I have the privilege of working in one of the few labs globally equipped with an MPI scanner—and the only one in Canada. This access positions us at the forefront of advancing this technology for in-vivo cell tracking applications.
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Zachary Freeman *(Feb/25, zfreema3@uwo.ca
Bachelor of Sciences, Honours in Health Sciences, Wilfrid Laurier University.
2nd year Masters of Science Candidate, Department of Epidemiology and Statistics, Schulich School of Medicine & Dentistry. Supervisor: Ana Lohmann MD PhD, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research examines the associations between body composition (obesity and sarcopenia) and prognostic markers in patients with metastatic breast cancer. This study aims to identify meaningful associations that could enhance patient screening and inform healthcare decisions by analyzing liquid biopsy data and prognostic blood biomarkers.
Key Techniques Used: As part of my research on body composition and prognostic markers in metastatic breast cancer, I used validated Python-based software to analyze muscle and adipose tissue areas from patient CT scans. Additionally, I have employed generalized linear models to statistically analyze continuous body composition data with circulating tumor cell (CTC) counts.
Why this project?: I chose this project because I have a personal connection to breast cancer survivors within my own family. I am motivated to participate in research that will improve care and treatment for all patients.
Why Western?: I chose Western University not only for its Master of Science in Epidemiology program and research but also for the many opportunities to engage in extracurricular activities.
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Anayra de Fatima Goncalves Santiago, *(April 17/25, agonca6@uwo.ca)
B.Sc. in Biological Sciences - Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
1st Year PhD Candidate, Department of Anatomy and Cell Biology, Schulich School of Medicine & Dentistry. Supervisor: Armen Parysan MD, PhD and Alison Allan PhD, Breast Cancer Canada Translational Research Unit, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: I'm using advanced techniques known as spatial omics to create detailed maps of the changes in molecules produced by cancer cells and nearby non-cancer cells within breast cancer tissue. My research aims to identify new biomarkers linked to the spread of triple-negative breast cancer (TNBC) to the axillary lymph nodes and its resistance to neoadjuvant therapy. Ultimately, these biomarkers could lead to more personalized treatment plans and the development of more effective drugs for TNBC patients.
Key Techniques: Spatial proteomics and transcriptomics, bioinformatics, immunohistochemistry, cell culture
Why This Project?: This project provides a valuable opportunity for me to develop new skills in bioinformatics and programming while contributing to discoveries in breast cancer biology that could improve patient outcomes.
Why Western?: I chose Western because of its reputation as a leading institution, its distinguished scientists and supervisors, and the opportunity to work with translational researchers who will enhance my professional development in science.
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Danial Hadi MBBS/MD *(Feb/25, Danial.Hadi@lhsc.on.ca)
Khyber Medical College. MRCPI, Royal College of Physicians of Ireland
Clinical Fellow, Medical Oncology, Verspeeten Family Cancer Centre, London Health Sciences Centre, and Schulich School of Medicine & Dentistry. Supervisor: John Lenehan MD and Saman Maleki PhD, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Its fascinating how Immune Checkpoint Inhibitors (ICIs) have transformed cancer care, but unfortunately, not all patients derive benefit, and some only do so for a short period of time. I'm part of an excellent team exploring the role of microbiome manipulation in trying to understand and overcome resistance to ICIs in patients with cancer. Our research focuses on studying Fecal Microbiota Transplantation (FMT) as an adjunct to ICIs in treating cancers. Whilst FMT has been around for decades, for treating conditions like Clostridium difficile infections and inflammatory bowel disease, its role in cancer care is an emerging area of research. Combining FMT with ICIs, represents a potential strategy in manipulating the microbiome to overcome resistance to ICIs.
Why this Project?: Investigating poor response/resistance to ICIs is a field of interest for me. The research team at LHSC has pioneered a novel approach to FMT with oral capsules, prepared with stool from healthy donors, and is being studied in multiple different cancer types. This research aligns perfectly with my passion and provides a unique opportunity for progress on the road to unlocking the cure for cancer!
Why Western?: Western University programs are focused on innovation and translational research, encouraging researchers to express their ideas with freedom, whilst providing an excellent overall environment to flourish.
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Oneeb Hassan (*Dec/24, ohassa2@uwo.ca)
BSc in Medical Sciences, Western University
2nd year Master's Degree in Department of Physiology and Pharmacology, Supervisor: Chris Pin PhD, Baker Center for Pancreatic Cancer, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Dysregulation of calcium (Ca2+) signaling is a critical factor in the development and progression of pancreatic cancer. Aberrant Ca2+ signaling through pathways like store-operated calcium entry (SOCE) leads to elevated cytosolic Ca2+ levels. This increase in cytosolic Ca2+ is implicated in several key processes, including proliferation, metastasis, and chemotherapy resistance in cancer cells. Our previous research has identified SPCA2C, a pancreas-specific protein, as a potential regulator of SOCE activation. To further elucidate its role in pancreatic pathology, we have generated a knockout mouse model and are currently investigating the impact of SPCA2C deficiency on pancreatic cancer initiation and progression.
Key Techniques Used: Live-cell calcium imaging, IF/IHC, RT-PCR and western blotting.
Why this project?: Pancreatic cancer is a highly lethal disease (5-year survival rate < 12%) that is growing in prevalence. Identifying novel therapeutic targets will hopefully increase patient survival.
Why Western:I spent time doing research in this lab during my undergrad and enjoyed the lab dynamic. Also, having the opportunity to work in the hospital and see patients who can benefit from my research was very motivating.
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Morgan He *(Jan/25, mhe52@uwo.ca)
MASc in Electrical and Computer Engineering, University of Waterloo and BSc in Electrical Engineering, University of California, San Diego
1st year PhD degree in the Department of Computer Science. Supervisor: Pingzhao Hu PhD, Western University
Project: My research involves developing artificial intelligence (AI) models to analyze medical datasets. I have focused on developing and using computing algorithms to help with tasks like detecting and highlighting cancer in medical images. For example, using several mammogram datasets, I have trained AI models to locate and segment tumors within the images. This work aimed to provide an additional tool for healthcare professionals, facilitating the interpretation of voluminous data. The objective was to streamline the interpretation process, potentially accelerating it and reducing human error. In addition, I am using large AI models to analyze Breast cancer histology datasets from The Cancer Genome Atlas Program (TCGA) to study the cancer gene mutation status. My research aims to incorporate new AI technology to address critical challenges in the medical field.
Why this project?: I chose this project to advance my skills and applications in the medical research field. My background was mainly in computer science and electronics and I have interest in using these to incorporate AI deep learning to assist in real world applications.
Why Western?: I chose Western University for the strong research program and to work with Dr. Pingzhao Hu in his lab. I am able to focus on incorporating machine learning and statistics for multimodal health data in precision medicine with multidisciplinary and collaborative team projects in AI and statistical genetics.
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Megan Hong *(Dec/24, mhong32@uwo.ca)
BSc. in Medical Sciences, Western University
4th Year PhD Candidate in Pathology & Laboratory Medicine, Supervisor: Saman Maleki PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Immune checkpoint inhibitors (ICIs) are immunotherapeutics that remove the “brakes” off the immune system to boost anti-tumour immune responses. One factor that influences the effectiveness of ICIs is the integrity of DNA mismatch repair (MMR) pathway in cancer cells. Defects in this pathway can promote T-cell activation, rendering tumours more susceptible to anti-tumour immune responses. In various cancers, patients with MMR-deficient tumours respond better to ICIs, such as anti-PD1 therapy, than those with MMR-proficient tumours. While anti-PD1 therapy is approved for patients with advanced MMR-deficient solid tumours primarily as second-line therapy, only 30-40% of these patients respond to treatment. This highlights the need to understand the mechanisms underlying resistance to anti-PD1 therapy and develop novel strategies to treat non-responding patients. However, it is unclear whether MMR deficiency can affect earlier stages of anti-tumour immune responses, such as the activity of innate immune cells like macrophages. Macrophages play a crucial role in shaping anti-tumour immune responses, as they can either promote or suppress T-cell activation. My research will use pre-clinical models to explore how MMR deficiency affects the phenotype and function of macrophages in tumours and how this can play a role in the response to various ICIs. These findings will provide valuable insight into mechanisms underlying ICI response in MMR-deficient tumours and enable the selection of appropriate ICI therapies for these patients. Furthermore, this can lead to the development of combination therapies that target both innate and adaptive immune responses to improve patient outcomes.
Key Techniques Used: Flow cytometry, immune cell/tissue culture, magnetic cell isolation, mouse handling, ELISA
Why this project?: I have always been interested in understanding the role of the immune system in the context of disease progression. This project offered an exciting opportunity to explore the intricate relationship between the host immune response and cancer, with the goal of developing more effective immunotherapies to improve patient outcomes.
Why Western?: I chose Western University for its strong research programs and research centers spanning on-campus and hospital sites, offering a unique opportunity to engage in cutting-edge translational research.
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Saqib Khan *(Jan/25, saqib.khan@lhsc.on.ca)
MD, Aga Khan University Hospital
Clinical Fellow, Western University- Verspeeten Family Cancer Centre (VFCC) at London Health Sciences Centre (LHSC). Supervisor, Daniel Breadner MD. FRCPC.
Project: My research focuses on transforming cancer care by optimizing treatment strategies through evidence-based innovation and a deeper understanding of clinical decision-making, mainly focusing on lung and upper gastrointestinal malignancies. I evaluate cutting-edge therapies, such as antibody-drug conjugates versus conventional agents like docetaxel, to improve outcomes in advanced non-small cell lung cancer. I am investigating treatment consistency and variability in esophageal and gastroesophageal cancers to uncover patterns influencing recovery, quality of life, and survival. My work also explores the factors shaping therapy choices, including why clinicians prefer certain immune checkpoint inhibitor-based regimens in advanced lung cancer. Beyond these efforts, I am leading innovative projects under grant review, including a comparison of systemic and chemo-radiotherapy strategies for locoregional esophageal tumors, a study on the safety and clinical impact of Moringa Oleifera to maintain nutritional status during treatment, and a mixed-methods investigation into how immunotherapy response predictions influence patient decisions in thoracic cancers, alongside with my supervisor. My research aims to refine treatment paradigms, improve outcomes, and empower patients in their cancer journey by bridging clinical insights with real-world practice.
Why this project?: I've always been drawn to thoracic cancers due to their complexity and the profound impact they have on patient's lives. These cancers are not only challenging to treat but also often diagnosed at advanced stages, making the need for innovative therapies even more pressing. The opportunity to improve treatment outcomes and enhance these patients' quality of life has always been a driving force in my research.
Why Western?: I chose Western University for its innovative research program in thoracic oncology, which is at the forefront of pioneering treatments and therapies. Its strong association with the Verspeeten Family Cancer Centre offers exceptional opportunities to collaborate with leading experts, access cutting-edge resources, and contribute to impactful clinical research, making it an ideal place to advance my expertise and drive meaningful contributions in cancer care.
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Sarah Konermann, *(March/25, skonerma@uwo.ca)
Honours Bachelor of Science in Physics, University of Ottawa. Master's in Medical Radiation Physics, McGill University.
1st year PhD Candidate, Department of Medical Biophysics, Schulich School of Medicine & Dentistry. Supervisor: Stewart Gaede PhD, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: I am researching a new application of radiation therapy, called cardiac radioablation. Cardiac radioablation is a treatment for ventricular tachycardia, a life-threatening heart condition. My work involves identifying which pre-treatment images are required to identify radiation targets in the heart and comparing different treatment types in order to identify which technique results in the best target coverage while minimizing damage to other organs. I am also working on creating a clinical cardiac radioablation program at the London Health Sciences Centre.
Key Techniques Used: Computed tomography (CT), Magnetic Resonance Imaging (MRI), radiation therapy
Why this project?: Cardiac radioablation is an up-and-coming treatment modality for ventricular tachycardia, with very promising early results. I chose this project because I want to be a part of this leading-edge treatment that appears to be able to save lives.
Why Western?: Though circumstances in my personal life led me to London, I am excited to study at Western University because of its leadership in the fields of imaging and radiation therapy. Western is truly a leader in my field of Medical Physics.
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Jasmine Lau *(April/25) jlau389@uwo.ca
4th year undergraduate, Bachelor of Medical Sciences (BMSc), Schulich School of Medicine & Dentistry, Western University. Supervisor, John Ronald PhD, Department of Biophysics, and Oncology, Roberts Research Institute, Schulich School of Medicine & Dentistry, Western University.
Project: Imaging reporter genes allow for non-invasive and longitudinal tracking of transplanted cells in vivo, allowing researchers to monitor tumour growth and metastasis and track cellular therapies (e.g. immunotherapies). However, common reporter genes are foreign to mice—altering normal immune responses and provoking rejection of transplanted cells when used in immunocompetent animals. My project aims to develop a novel Magnetic Resonance Imaging (MRI) reporter gene for cell tracking in mice with a fully functioning immune system, allowing researchers to more accurately recapitulate the tumour microenvironment in preclinical models and hopefully improve the translation of therapeutics to humans.
Why this Project?: Being able to visualize and track cells throughout the body using reporter genes seemed so fascinating to me, as it could answer critical questions about cancer behaviour (growth, metastasis, response to therapies) and be used to evaluate new cellular therapies (whether they accurately target tumours, have off-target side effects, and how long they persist). This project specifically involved developing a new reporter gene tool to improve cancer preclinical models, which could have a large impact, and also provided an exciting opportunity to learn a wide range of lab skills.
Key Techniques Used: Molecular cloning, cell culture, flow cytometry, immunofluorescence staining, Magnetic Resonance Imaging (MRI), image analysis, bioluminescence imaging (BLI).
Why Western?: I chose Western University because of their strong dedication to research and mentorship, even for undergraduate students through the Scholar’s Electives program. I also value the warm and welcoming community and collaborative campus environment.
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Stephanie Leighton *(Feb/25, sleighto@uwo.ca)
HBSc. Chemistry and Biology for Health Science, University of Toronto
4th year PhD Candidate, Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry. Supervisors: Dale Laird PhD and Silvia Penuela PhD.
Project: My research focuses on characterizing the role of PANX1, a channel-forming glycoprotein that is upregulated in many cancer types, including melanoma. Using a CRISPR-Cas9 targeting strategy and pharmacological PANX1 blockers, we are investigating how disruptions to the PANX1 signaling network contribute to the malignant phenotype of melanoma in vitro and in xenograft model systems. Our investigations aim to understand the role of PANX1 and uncover novel therapeutic strategies for targeting PANX1, potentially leading to improved treatment options for patients with melanoma and other cancers in which PANX1 is disrupted.
Key Techniques Used: Immunofluorescence microscopy, live cell imaging, immunohistochemistry, tissue culture, western blotting, RT qPCR, biochemical assays, computational modeling, and RNA sequencing.
Why This Project: As a childhood cancer survivor, I’m deeply passionate about cancer biology and advancing our understanding of various oncogenic signaling pathways. My project employs a diverse range of research techniques to investigate the role of PANX1 in melanoma, with the goal of driving translational advances in cancer treatments that will one day make a difference for others.
Why Western?: I chose Western University for its emphasis on interdisciplinary research and its collaborative environment that supports both research advancement and leadership development.
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Alana Lopes *(Feb/25, alopes27@uwo.ca)
BSc. in Medical and Biological Physics, McMaster University
2nd Year PhD Candidate, Department of Medical Biophysics, Schulich School of Medicine & Dentistry. Supervisors: Aaron Ward PhD and Matthew Ceccini MD PhD.
Project: My research focuses on integrating eye tracking technology with machine learning models in digital pathology to develop tools that will help pathologists in their assessment of cancer. Specifically, we are developing an artificial intelligence assistant that will use pathologists' eye gaze and voice to identify suspicious regions in pathology images, thus bridging the gap between humans and computers in a digital pathology workflow.
Why this project?: The field of pathology is undergoing a digital revolution and I am excited to be one of the researchers working to develop solutions in this field that will support pathologists and help lead to better treatment outcomes for cancer patients.
Key Techniques Used: Eye tracking, machine learning, whole slide image processing, voice recognition algorithms
Why Western?: Dr. Ward's research program being directly integrated within the cancer center offers a unique opportunity for clinical collaboration. The benefit of being able to develop our research projects alongside clinicians helps to ensure our research stays focused towards addressing the most pressing clinical needs.
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Katelyn MacNeil *(Dec/24, kmacne9@uwo.ca)
BSc. in Microbiology & Immunology and Neuroscience, Dalhousie University, NS
6th year PhD Candidate in Microbiology & Immunology, Supervisor: Joe Mymryk PhD, Head & Neck Translational Cancer Unit, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: The star of my research project is human adenovirus, which has the distinction as the first human virus shown to cause cancer, albeit in a rodent model. While there are no examples of adenoviruses as causative agents of human cancers, studying how the viral oncogene E1A, produced during adenovirus infection, regulates gene expression has provided invaluable insights into cancer development. In particular, I focus on how E1A hijacks two of our cellular proteins, CDK7 and CDK9, to promote viral gene expression. These findings on what the E1A oncoprotein targets could also inform us about pathways relevant to cancer.
Key Techniques Used: Co-immunoprecipitation, qPCR, luciferase assays, and western blot analysis.
Why This project?: I loved that this project would give me the opportunity to learn a variety of different research techniques and that it combined elements of virology, biochemistry, and genetics.
Why Western?: I came to Western University for Dr. Mymryk’s research program because I liked that it focused on the many different targets of the viral E1A oncoprotein.
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Agetha Mahendran, *(Apr/25, amahen@uwo.ca)
BMSc, Honours Specialization in Biochemistry of Infection and Immunity, Western University.
2nd year PhD Candidate, Department of Microbiology and Immulogy, Schulich School of Medicine & Dentistry, Western University. Supervisor: S.M. Mansour Haerfyar, PhD.
Project: During chronic stress, communication between the nervous and immune systems can become disrupted, reducing the anti-tumor response by the immune system. Our previously published work revealed that glucocorticoids, a class of stress-related hormones, weaken the function of innate-like T cells. As a result, these cells fail to protect against cancers such as melanoma. My research aims to investigate the link between intracellular glucocorticoid receptor signalling and immune dysfunction in innate-like T cells with the aim of finding targetable molecules in these axes.
Key Techniques: mouse models, cell culture, flow cytometry, scRNA sequencing, qPCR
Why The Project?: I chose this project for its unique intersection of multiple disciplines, immunology and neuroscience, as well as its broad therapeutic applications.
Why Western?: I chose Western University for my graduate studies purely because of Dr. Haeryfar's research program. I enjoyed the diverse range of projects in our lab and the skills I could learn during the course of my PhD.
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Julia Mason *(Jan/25, jmason49@uwo.ca)
BSc. in Chemical Biology, McMaster University
6th year PhD Candidate, Department of Chemistry, Schulich School of Medicine & Dentistry, Western University. Supervisor: Len Luyt PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research focuses on the development of novel peptide-targeting imaging agents for Positron Emission Tomography (PET) imaging of specific cell receptors over-expressed in a variety of cancer types, more specifically prostate cancer. These projects have focused on the synthesis of peptide libraries to optimize their targeting ability and improve biodistribution. These peptides incorporate a fluorine-containing small-molecule which can be radiolabelled with a radioactive fluorine-18 atom, used specifically for PET imaging. PET imaging allows for non-invasive imaging to locate cancer biomarkers to provide accurate and early diagnoses and patient stratification for therapy.
Key Techniques Used: Small molecule synthesis, solid-phase peptide synthesis, high-performance liquid chromatography, bioassays, cell culture, radiochemistry, nuclear magnetic resonance spectroscopy, mass spectroscopy.
Why this Project?: I originally chose this project to be able to synthesize novel compounds and run their initial evaluation toward the development of targeted cancer imaging agents. Like most people, cancer has affected many family members, and I wanted to play a role in progressing cancer research, where this project would enable detection and treatment monitoring. I quickly dove into the exciting world of radiochemistry and the field's wonderful applications. It is a privilege to be part of this growing field, continuously seeing media headlines of new radiotherapeutics in clinical trials and patient treatment.
Why Western?: I chose Western University for its research programs, in particular having the opportunity to advance my skills in Dr. Luyt's lab. While the research is chemistry focused, its interdisciplinary nature allows students to establish a wide range of skills from small molecule synthesis to bioassays to animal imaging.
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Kasha Mansour *(Feb/25, kmansou@uwo.ca)
Bachelor of Medical Science with Honours Specialization in Medical Cell Biology, Western University
4th year PhD Candidate, Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry. Supervisor: Natalie Bérubé PhD, Children's Institute for Health Research, London Health Sciences Centre.
Project: My research is focused on generating and characterizing a genetic knockout mouse model of astrocytoma, which is a form of brain cancer. I hope to be able to use this model to gain insight into the mechanisms that govern tumourigenesis, identifying pathways that are altered and then testing drugs against these targets. Given the current lack of targeted therapies for the treatment of brain cancer I anticipate that this model will be a useful tool to help with the discovery of new treatments.
Key Techniques Used: Key techniques that I have used/plan on using include mouse models, immunohistochemistry, single cell sequencing, and cell culture.
Why this project?: I chose this project because this is an exciting tool that I believe can be useful for cancer research in the future. Additionally, my family has been affected by cancer, and I wanted to be able to contribute to finding new treatments to provide hope to patients.
Why Western?: I chose Western University because of the strong research community which provides many opportunities for collaborations. There are a wide range of researchers with different skills and equipment that can be used to produce top quality research.
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Zhaocheng Nie *(Jan/25, znie25@uwo.ca)
BSc, Honours specialization in Genetics and Biochemistry, Western University
4th year PhD Candidate, Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University. Supervisor: Murray Junop PhD, Western University.
Project: Interstrand crosslink (ICL) is a covalent linkage formed between two opposing strands of DNA, and by linking the two DNA strands it prevents strand separation, hinders essential cellular processes, and eventually leads to cell death. The ICL-damaged part of the DNA needs to be properly repaired, and SNM1A is one of the nucleases implicated to be involved in the ICL repair pathway. More interestingly, SNM1A can perform two different nuclease activities (exonuclease and endonuclease activity) using the same active site, which makes it challenging and also fascinating to learn about its exact role in the repair process. Understanding SNM1A and in general the ICL repair pathway will greatly contribute to improving cancer therapies that involve using ICL-inducing agents to target rapidly proliferating cancer cells.
Why this project?: The clinical problem presented here is something that can be simply stated, but remains complicated to solve. The fact that this seemingly "obvious" problem lacked any effective, low-cost solution told me that this would be an exciting project to navigate throughout my PhD.
Why Western?: Western offers one of the few CAMPEP accredited PhD programs in Ontario, which will enable me to continue to pursue a career as a medical physicist. Additionally, the program at Western allowed me to conduct research directly in the cancer centre, providing more hands-on opportunities to learn about how we treat patients here in London.
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Kaitlyn Palmateer *(April/25) kpalmat@uwo.ca
BMSc in Biochemistry of Infection and Immunity, Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University.
1st year Master's Candidate, Department of Microbiology & Immunology Schulich School of Medicine & Dentistry, Western University. Supervisor, Joe Mymryk PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre.
Project?: My research focuses on human adenovirus E1A protein, which is essential for infection with human adenovirus. I study how this protein interacts with various proteins in human cells during infection. Specifically, I am studying the interaction between viral E1A and host PKA, which is a protein essential for cell signaling. I am making mutant viruses that have lost the ability to make these interactions and evaluating how the cellular environment changes during infection with these viruses. I hope to understand how these virus-host interactions might help the virus to evade the host immune system.
Why this Project?: I was already familiar with human adenovirus from my undergraduate work, and had found E1A research interesting. I chose this project because it gives me the opportunity to use a variety of techniques in areas including virology and biochemistry.
Key Techniques Used: I plan on primarily using cell culture, western blotting, fluorescence microscopy and RNA-seq.
Why Western?: I chose Western University because I enjoyed my research experience here during my undergraduate studies. I saw continuing my studies at Western as an opportunity to continue working in a department with a wide variety of research interests.
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Nachuan Pan, *(March/25, npan9@uwo.ca)
BMSc Honours Specialization Program, Department of Pathology & Laboratory Medicine, Schulich School of Medicine & Dentistry, Western University.
2nd year Master's Candidate, Department of Otolaryngology. Supervisor: Anthony NIchols MD PhD, Translational Head & Neck Cancer Research Laboratory, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Our lab is studying the application of a novel targeted therapy drug called naporafenib in treating anaplastic thyroid carcinoma (ATC). Some preliminary evidence has suggested this drug's excellent therapeutic potential, allowing us to incorporate immunotherapy to enhance the anti-cancer effects. Therefore, we are currently investigating the combination of targeted therapy and immunotherapy in the context of ATC and we hope to translate our knowledge into clinical practice soon!
Key Techniques Used:Mouse model, Cell culture, In vitro drug screening
Why this project?: I chose this project because I believe in the synergy between different anti-cancer treatment and it can bring a new perspective to the current clinical practices.
Why Western?: I completed my undergraduate degree at Western and the support and mentorship I received were very critical to my success. I chose to continue my graduate study here because of the same reason.
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Urvi Patel *(Mar/25, upatel32@uwo.ca)
BMSc with Honours Specialization, Department of Biochemistry and Cancer Biology, Schulich School of Medicine & Dentistry, Western University.
3rd Year PhD Candidate, Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Alison Allan PhD, Breast Cancer Canada Translational Research Unit, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research focuses on exploring why the triple negative subtype of breast cancer metastasizes to the lung. Currently, there is evidence that breast cancer cells release small particles called extracellular vesicles which travel to the lung and make changes to form a favourable environment for metastasis. Therefore, I will be looking at the changes these extracellular vesicles make in the lung and whether we can inhibit them with a drug, called tipifarnib, to delay metastasis. This research will help to us better understand lung metastasis in breast cancer patients so we can identify new biomarkers and determine whether targeting extracellular vesicles is a potential therapeutic strategy for triple negative patients.
Why this project?: I've always been interested in cancer research which led me to take on this project during my undergrad. I enjoyed learning about the mechanisms of metastasis and have been able to continue to expand my understanding through this project.
Key Techniques: Cell culture, immunoblotting, extracellular vesicle isolation, cell-based assays
Why Western?: I chose Western because I really enjoyed being part of a supportive lab environment and received great mentorship throughout my project. Western has a strong academic community and I look forward to collaborating with other researchers.
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Robert Policelli *(Mar/25, rpolicel@uwo.ca)
Undergraduate degree: Honours Specialization in Medical Biophysics and Biochemistry, Schulich School of Medicine & Dentistry, Western University.
2nd year Master's Candidate, Department of Medical Biophysics, Schulich School of Medicine & Dentistry, Western University. Supervisor: Aaron Ward PhD, Gerald C. Baines' Centre for Translational Cancer Research, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research aims to find predictors of treatment success for patients with brain tumours. I look at patient imaging (e.g. MRI, CT), patient characteristics (e.g. age, sex), and radiotherapy treatment parameters (e.g. amount of dose delivered) to attempt to find patterns between patient who have successful versus unsuccessful treatments. I also use AI to attempt to find predictors that are unseen by the human eye. I hope that these predictors can be used to ensure each patient receives the the best treatment possible to optimize success and patient quality of life.
Why this project?: I choose this project due to the passion by both the clinicians and researchers to solve this problem. The collaboration with clinicians provides a unique opportunity to work directly towards implementing these predictors in patient care to improve treatment planning.
Key Techniques: I create AI models and use coding to help analyze the patterns between patients with successful and unsuccessful treatment. I also use statistics to demonstrate the significance of the predictors in predicting treatment outcomes. Most importantly, I collaborate with clinicians to attempt to work towards finding the most applicable predictors for the work they are doing and ensure that they can be used effectively to help patient treatment planning.
Why Western?: I choose Western University due to the amazing collaboration between researchers and clinicians. The high level of communication and teamwork between all scientists helps push projects forward towards improving patient care.
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Joana Ribeiro Pinto *(Mar/25, jribeir7@uwo.ca)
Bachelor in Science in Physiology and Functional Genomics from Aix-Marseille University, France.
Master in Science in Genomics and Data Analysis from Aix-Marseille University, France.
3rd Year PhD Candidate, Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Christopher Pin PhD, Baker Centre for Pancreatic Cancer, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research focuses on understanding how changes in DNA methylation - chemical markers that control gene activity - play a role in pancreatic cancer. Using patient-derived organoids, which are 3D miniature tumour models, we aim to map DNA methylation patterns linked to different pancreatic cancer subtypes. Since these subtypes vary in their aggressiveness and response to treatment, identifying specific patterns will help develop better diagnostic and prognostic tools with real clinical impact.
Why this project?: Pancreatic cancer is a highly fatal disease with a 5-year survival rate around 12%, partly due to its high heterogeneity and its rapid adaptability to external factors. Identifying stable markers for better patient management and novel therapeutic targets is crucial to improve patient survival.
Key Techniques: Purification of nucleic acids (DNA and RNA);DNA methylation array (Human Methylation EPIC v2 array);Real time polymerase chain reaction (RT-qPCR);Bioinformatic analysis (R).
Why Western?: I chose Western University to work with Dr Pin, whose renowned research on pancreatic cancer brought me to Canada and to his lab. Working in the Verspeeten Family Cancer Centre is both humbling and motivating, as walking past patients each day reminds me who I am fighting for and the real impact my research can have.
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Noor Rizvi *(Feb/25, mrizvi9@uwo.ca)
Bachelor of Life Sciences Honors, Queen University
2nd year MSc. Candidate, Department of BioChemistry, Western University. Supervisor: Vanessa Dumeaux, PhD.
Project: Our study focuses on predicting the behaviour of Ductal Carcinoma in Situ, a non-invasive breast cancer. We aim to identify genetic markers that may predict disease aggression, response to radiation, and the likelihood of recurrence. This could enable personalized treatment plans, reduce unnecessary intervention, and identify alternatives for those resistant to current therapies.
Key Techniques Used: My research primarily involves computational techniques, such as data analysis using statistical models and bioinformatics tools for data processing.
Why this Project?: I’ve always been passionate about breast cancer research. Working on projects like Every Breast Counts with Women’s College Hospital introduced me to the power of advocacy in improving cancer outcomes. But beyond advocacy, I am equally drawn to the research that drives meaningful change behind the scenes. That’s what excites me most about this project—it allows me to build upon that advocacy work and contribute to research that, ultimately, can lead to advancements in personalized medicine and improved health outcomes for all breast cancer patients,
Why Western?: I chose UWO because of the amazing research opportunities in cancer studies and the chance to be part of a meaningful project. Plus, the beautiful campus and great facilities made it an easy choice!
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Fatima Sántillán Espinoza *(Mar/25, fsantill@uwo.ca)
Bachelor of Science (Chemistry), Universidad Nacional de Ingenieria, Peru. Master of Science, Pontifical Catholic University of Peru. Master of Science, Collaborative Specialization in Molecular Imaging, Schulich School of Medicine & Dentistry, Western University.
2nd Year PhD Candidate, Department of Chemistry, Western University. Supervisor: Len Luyt PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project:I develop peptide-based nanomaterials for various biomedical applications, including cancer imaging, drug delivery, and theranostics.
Key Techniques: Field emission scanning electron microscopy, confocal fluorescence microscopy, solid-phase peptide synthesis, circular dichroism spectroscopy, rheology, antibacterial assays, Fourier-transform infrared spectroscopy, raman spectroscopy, high-performance liquid chromatography, mass spectrometry, cell culture, cell viability, drug delivery.
Jeri Spilberg *(Feb/25, jspilber@uwo.ca)
BSc. Molecular Biology and Genetics, Honours, University of Guelph
1st year Master's Candidate, Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Alison Allan PhD, Breast Cancer Canada Translational Research Unit, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Breast cancer is currently the second leading cause of death in Canadian women. The majority of these deaths occur due to metastasis to organs such as the lungs, liver, brain and bone. Metastasis is the spread of cancer cells from the initial site/organ to a secondary site/organ and can be characterized as localized, regional, or distant. Previous research has shown that when cancer cells metastasize, they tend to migrate to specific organs, known as organotropism. I am currently focusing on studying integrin expression in breast cancer cells. Integrins are crucial cell adhesion proteins that enable cells to bind with each other and with the extracellular matrix. This research is particularly significant because preliminary data suggest that integrins may play a role in driving breast cancer metastasis. The hope is that learning more about the role of integrins in metastasis will contribute to a better understanding of metastatic mechanisms and aid in developing stronger treatments.
Key Techniques Used: Cell culture in normal and hypoxic conditions, Chemicon Alpha/Beta integrin-mediated cell adhesion assay.
Why this Project?: Cancer biology has always been a big interest of mine, and when the opportunity to work on a project that will advance cancer research arose, I jumped at the opportunity. This research will provide a greater understanding of the role that integrins play in organ-specific metastasis and provide a strong foundation for additional pre-clinical in vivo studies. Overall, I hope this research will help the future of breast cancer treatment.
Why Western?: The vibrant community, academic prestige, and lab that I'm working in were tremendous factors in my decision. I am proud to be a Mustang and look forward to my time here!
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Aswin Sureshkumar *(Feb/25, asuresh6@uwo.ca)
Bachelor of Medical Sciences, Honours Interdisciplinary Medical Sciences, Department of Medical Sciences, Schulich School of Medicine & Dentistry.
4th year PhD Candidate, Department of Physiology & Pharmacology, Schulich School of Medicine & Dentistry. Supervisor: Christopher Pin PhD, Baker Centre for Pancreatic Cancer, London Health Sciences Centre.
Project: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths, largely due to late detection and chemotherapy resistance. Mutations in the KRAS gene are found in 97% of PDAC cases, but additional factors like ATRX loss are needed to initiate the disease. In mouse models, ATRX deficiency combined with KRAS mutations caused more severe pancreatic damage in females. At the same time, males showed less damage and retained healthier acinar cells, suggesting a sex-specific role in PDAC progression. These findings highlight ATRX as a potential target for early detection and therapy, with further research planned using patient-derived cells and organoids to explore its mechanisms.
Key Techniques Used: Pancreatic tumour organoids, PDAC patient-derived cell lines, IHC/IF, RNA-sequencing, mouse models, flow cytometry, CRISPR-Cas9
Why this Project?: Investigating pancreatic cancer through epigenetics provides a unique opportunity to explore gene regulation beyond genetic mutations, paving the way for innovative targeted therapies and enhanced early detection methods by bridging modern cancer research techniques with clinical care. Since epigenetic changes are reversible, this field provides a promising avenue for advancing personalized medicine and developing new treatments for one of the most lethal cancers.
Why Western?: I completed my undergraduate studies at UWO, where I became well-acquainted with the institution’s strong reputation for academic and research excellence, as well as its culture of mentorship and collaboration. I am particularly inspired by Dr. Pin's genuine commitment to advancing PDAC research through a strategic integration of progressive laboratory techniques and a targeted scientific approach. His dedication to continuously refining hypotheses based on emerging data fosters an environment where meaningful discoveries can be made, driving progress in understanding this complex disease.
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Emily Tomas, *(April 17/25, etomas3@uwo.ca)
Bachelor of Medical Sciences (BMSc) program, Western University.
5th year PhD Candidate, Bachelor of Medical Sciences (BMSc) program, Department of Anatomy and Cell Biology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Trevor Shepherd PhD, The Mary & John Knight Translational Ovarian Cancer Research Unit, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: My research focuses on characterizing 3D models to study epithelial ovarian cancer metastasis. I hope to determine the biological similarities and differences between cells as they metastasize and as they grow into tumours using the "spheroid" and "organoid" model systems, respectively. This research will help us to understand this disease at different stages and how to best treat patients moving forward.
Key Techniques Used: 3D spheroid model; 3D organoid model; immunoblotting; RT-qPCR; immunofluorescent imaging; immunohistochemistry; RNA-sequencing.
Why This Project?: Early on, I was always interested in studying cancer biology. This project provided me the opportunity to help establish this new model system (i.e. organoids) in our facility and advance research in this field.
Why Western?: I chose Western University way back for my undergraduate degree because of it's amazing medical sciences program. After learning about the many research opportunities and collaborative environment, I chose to stay longer!
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Alfredo Varela Vega *(April/25) avarelav@uwo.ca
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Edward Wang *(Feb/25, ewang225@uwo.ca)
BASc in Chemical and Biological Engineering, University of British Columbia.
5th year MD/PhD Program, 3rd year PhD, Department of Biomedical Biophysics, Schulich School of Medicine & Dentistry. Supervisors: Sarah Mattonen PhD and Pencilla Lang MD PhD, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: Radiation therapy is an effective form of cancer treatment. Advances in technology now allow doctors to utilize high dose radiation in treating multiple tumour sites in patients with advanced cancer. Creating a safe and effective radiation treatment plan can be a time-consuming process, especially for complex treatment of multiple targets. In our work, we are developing artificial intelligence models to help radiation oncologists decide what treatment to prescribe, and to automate parts of the treatment planning pipeline.
Key Techniques Used: We primarily develop generative artificial intelligence models which are mostly coded in the Python programming language. Our data consists of medical images acquires from patients undergoing treatment.
Why this Project?: My clinical interest is oncology, and my technical/scientific interest is artificial intelligence. This project is a perfect fit!
Why Western?: I chose Western for the opportunity to work with Dr. Mattonen. Our lab is located inside the cancer centre, which enables very close collaboration with the the clinical team, ensuring that our work is always focused towards true unmet clinical needs.
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Nicole Wang *(Mar 25/nwang268@uwo.ca)
Bachelor of Medical Sciences with Honours Specialization in Microbiology and Immunology, Western University.
3rd year PhD Candidate, Department of Microbiology & Immunology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Mansour Haeryfar PhD
Project: My research focuses on understanding the role of a unique type of immune cell, MAIT cells, in ovarian cancer. MAIT cells have been shown to either promote or inhibit cancer progression, depending on the cancer type, but their behavior in ovarian cancer remains unclear. I am using various tools to investigate this, including a mouse cancer model, patient samples, and a bioinformatic dataset. By gaining a deeper understanding of how MAIT cells function in ovarian cancer, I hope to inform new strategies for targeting them for cancer immunotherapy.
Why this project?: I chose this project because I believe MAIT cells have great potential for cancer immunotherapy.
Key Techniques: Mouse cancer models, mouse and human tissue processing, flow cytometry, cell culture, single-cell RNA-sequencing analysis, qPCR.
Why Western?: I did my undergraduate degree at Western, where I completed my undergraduate thesis in the Haeryfar Lab. I loved the people and the research so much, so I decided to call Western home for a few more years!
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Jack Webb *(Dec/24, jwebb47@uwo.ca)
BMSc, Pathology & Pharmacology, Western University
4th year PhD Candidate, Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, Western University. Supervisor: Trevor Shepherd PhD, Translational Ovarian Cancer Research Unit, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: research focuses on epithelial ovarian cancer, a deadly disease often diagnosed late and resistant to chemotherapy. I study ULK1, a protein crucial for cancer cell survival and spread, as it regulates autophagy, disease progression, and mitochondrial health. By targeting ULK1, we aim to uncover new therapies that disrupt cancer cells' survival mechanisms, slow their spread, and improve treatment outcomes.
Key Techniques Used: Molecular analysis, drug treatments, fluorescence/luminescence assays, proteomics analysis, fluorescence reporters, transfections, 3D cell culture
Why this project?: My passion for understanding cancer biology and ovarian cancer cell survival and spread inspired me to expand on our lab's previous findings and further explore ULK1’s role in ovarian cancer progression and discover new treatment strategies.
Why Western?: I chose Western University for its exceptional research opportunities and supportive mentorship, which have been pivotal in advancing my ovarian cancer studies and developing my skills as an educator and academic leader.
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Alyssa Wu *(Jan/25, awu344@uwo.ca)
BSc. Life Sciences (Honours), McMaster University
1st year PhD Candidate, Department of Biochemistry. Supervisor, Shawn Li PhD, Department of Biochemistry, and Oncology, Schulich School of Medicine & Dentistry, Western University.
Project: My research investigates STAT3, a key transcription factor in cancer, using a comprehensive multi-omic approach to understand its role in tumour progression. Through CRISPR-Cas9-mediated STAT3 knockouts in various cancer cell lines, we analyze changes in protein expression, phosphorylation patterns, and secreted factors using advanced mass spectrometry techniques. Our studies reveal that STAT3 regulates far more targets than previously known and influences crucial cancer-related processes such as epithelial-mesenchymal transition (EMT), cell adhesion, and immune response. By combining proteomics, phosphoproteomics, secretomics, and spatial analysis of the tumour microenvironment, we aim to uncover novel STAT3-regulated pathways and identify potential therapeutic targets for cancer treatment. This research is particularly significant as it explores compensatory mechanisms activated in STAT3's absence, providing insights that could lead to more effective cancer therapies.
Key Techniques Used: Mass spectrometry-based proteomics and phosphoproteomics, bioinformatics analyses (differential expression, pathway enrichment analysis and protein-protein interaction network mapping), large-scale database mining, 2D/3D cell culture, immunofluorescence microscopy, immunohistochemistry, western blots, ELISA, RT-qPCR.
Why this Project?: Motivated by critical knowledge gaps in cancer biology, I chose this project to uncover the intricate signalling underlying common oncogenic pathways using cutting-edge technologies, aiming to develop more effective targeted therapies. This research not only advances our understanding of cancer mechanisms but also challenges me to effectively communicate complex scientific concepts that could ultimately improve patient care.
Why Western?: Western University offers exceptional opportunities to study cancer biology through hands-on experience with cutting-edge mass spectrometry and molecular biology techniques, perfectly aligning with my interest in understanding how post-translational modifications and oncogenic signalling pathways drive cancer progression at the molecular level.
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George Xie *(Feb/25, gxie27@uwo.ca)
BMSc, Honours Specialization in Biochemistry of Infection and Immunity, Western University.
1year PhD Candidate, Department of BioChemistry, Western University. Supervisors: Kun Ping Lu PhD and Xiao Zhen Zhou PhD
Project: My research focuses on the development of Pin1 inhibitors for cancer therapy, notably in aggressive cancers such as triple-negative breast cancer, glioblastoma, and pancreatic ductal adenocarcinoma. Previous research in our lab has shown that inhibition of Pin1 in combination with currently approved standard therapies can render these aggressive cancers eradicable by overcoming drug resistance pathways. Through structure and activity guided drug design, we aim to develop novel more potent and specific Pin1 inhibitors, then to apply these inhibitors in various cancer models to investigate their anticancer effects.
Key Techniques Used: Western Blot, Fluorescence Polarization, Immunofluorescence Microscopy, RT-qPCR.
Why This Project?:When I first joined the lab for my undergraduate thesis, I was immediately drawn to the Pin1 protein, discovered by my supervisors, and its role in so many diseases, including Alzheimer's Disease, cancer, and even viral infection. Given its high therapeutic potential and applicability, I am very excited to work on this project.
Why Western?: I chose Western University for its strong research program, notably working with my supervisors Dr. Kun Ping Lu and Dr. Xiao Zhen Zhou. Since our lab researches cancer, neurodegeneration, and infection, I'm able to gain experience in many fields, and have abundant opportunities for collaboration with the other cutting-edge research taking place at Western.
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Timothy Yau *(Jan/25, timothy.yau@lhsc.on.ca)
BMSc. in Medical Sciences at Western University
4th Year PhD Candidate in Medical Biophysics, Schulich School of Medicine & Dentistry, Western University. Supervisor: Stewart Gaede PhD, Verspeeten Family Cancer Centre, London Health Sciences Centre.
Project: For lung cancer patients, tumours are known to move with patient breathing. When treating cancers with radiation, this motion needs to be accounted for to ensure we do not uncessarily irradiate the surrounding healthy organs and tissues. My research focuses on the development of treatment techniques that would enable our machines to have the radiation beam track and follow the tumour during the treatment. This would allow for a more percise radiation delivery that would minimize treatment side effects and increase patient outcomes.
Key Techniques Used: 4D-CT and wide-volume dynamic CT (ie. volumetric 4D-CT) for lung tumour motion assessment, generative AI (ie. GAN models) for tumour tracking on x-ray images, biomechanical models (finite element modelling of the lung) for tumour tracking and time-series AI (RNN models for tumour motion prediction) for tumour tracking prediction from only surface detection, and external beam radiotherapy (VMAT, IMRT, DCA, etc.) for treatment delivery techniques.
Why this project?: The clinical problem presented here is something that can be simply stated, but remains complicated to solve. The fact that this seemingly "obvious" problem lacked any effective, low-cost solution told me that this would be an exciting project to navigate throughout my PhD.
Why Western?: Western offers one of the few CAMPEP accredited PhD programs in Ontario, which will enable me to continue to pursue a career as a medical physicist. Additionally, the program at Western allowed me to conduct research directly in the cancer centre, providing more hands-on opportunities to learn about how we treat patients here in London.
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Komila Zakirova (*Jan/25, kzakirov@uwo.ca)
HBSc in Molecular Biology and Genetics, University of Guelph
6th year PhD Candidate, Department of Pathology & Laboratory Medicine, Schulich School of Medicine & Dentistry, Western University. Supervisor: Fred Dick PhD, Cancer Research Laboratory Program, Verspeeten Family Cancer Centre, London Health Sciences Centre.
My project: My research focuses on the dormancy and spread of ovarian cancer spheroids, which are three-dimensional clusters of cancer cells. These spheroids are the primary cause of treatment resistance and disease relapse in patients diagnosed with high-grade serous carcinoma (HGSC). I investigate the molecular mechanisms that govern spheroid dormancy, with a particular focus on the roles of Netrin and Wnt signalling pathways. Our lab has identified these pathways as crucial for spheroid survival through genome-wide CRISPR screenings conducted in HGSC cell lines. To establish the function of survival genes from these pathways, I employ CRISPR-mediated gene editing alongside the cell culture model of dormancy that allows the replication of spheroid formation in vitro, similar to what is observed in ovarian cancer patients. Additionally, I perform xenograft experiments in immunocompromised mice to examine the dissemination of cancer cells and to model responses to therapeutic agents, enhancing our understanding of tumour behaviour and treatment effectiveness in vivo. Ultimately, my research aims to discover new avenues for developing targeted therapies to treat ovarian cancer and improve patient outcomes.
Why this project?: I have always been interested in the field of cancer biology, and this project offers an excellent opportunity to learn molecular biology techniques and enhance my critical thinking skills, which are essential for my development as a scientist.
Why Western?: I chose Western University primarily because of its innovative research programmes. I was particularly drawn to the opportunity to engage in advanced research in Dr. Dick’s lab, where pioneering projects and collaboration foster a dynamic environment for both academic and professional growth.
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