The London Regional Proteomics Centre (LRPC) comprises six core facilities operated through the Department of Biochemistry, in the Schulich School of Medicine & Dentistry at Western University.
These constituent core facilities provide service and instrumentation and will work cooperatively to fulfill your research needs in the area of proteomics, protein structure and protein function.
The FPF is the most common entry point for researchers requiring analysis of complex mixtures of proteins, i.e. proteomics. Equipment is available for the fractionation of these complex biological mixtures using chromatographic and electrophoretic procedures and for the preparation of protein spots or fractions for identification/analysis. Normally, samples will then be passed on to the appropriate mass spectrometry core facility for that purpose.
The BMSL features mass spectrometers of several types. These instruments allow the detailed analysis of protein mass and peptide composition through various approaches. Some of the works performed at BMSL include: mass determination of polar and/or high molecular weight molecules including peptides, proteins, glycyoproteins, etc.; sequence determination of peptides and peptides from proteins digests; protein identification in a mixture; and types and sites of protein modifications.
The Biomolecular NMR Facility houses state-of-the-art nuclear magnetic resonance (NMR) instrumentation and provides expertise required for the characterization of proteins, nucleic acids, carbohydrates and small molecules at the atomic level. A large number of biological NMR experiments and analyses software are available allowing the three-dimensional structure of proteins to be determined in solution, detailed characterization of protein-protein and protein-ligand interactions, and the identification of conformational changes, protein motions (dynamics) and folding processes.
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The MCF provides equipment, supplies, and expertise essential for determination of the three-dimensional structures of purified proteins through X-ray crystallography. Equipment includes a crystallization robot, an X-ray generator, and an area detector. Advice and technical assistance in sample preparation and experimentation is provided as needed for clients who wish to conduct the work themselves.COVID-19 Information for users of the LRPC
How We Can Help
LRPC facilities are accessible to users from Western University, external academic communities, and industrial settings. Users can perform their own sample analyses or use the services of qualified facility staff.
For further information on how the core facilities of the LRPC can accommodate your research goals, please contact us by email at: BioCORE@uwo.ca
